Kucherenko I. Development of electrochemical enzyme biosensors for the determination of ATP concentrations and creatine kinase activity

The thesis is dedicated to the development and optimization of the electrochemical biosensors based on immobilized enzymes for the determination of adenosine triphosphate (ATP), glucose, and creatine kinase. Working prototypes of monobiosensors and a biosensor system for the simultaneous determination of ATP and glucose were created. For the first time a conductometric biosensor based on hexokinase for determination of ATP and glucose was created. Analytical characteristics of the biosensors were studied and optimized. Influence of the working buffer parameters (concentration of magnesium ions, ionic strength, and buffer capacity) and different conditions of the biosensor storage and functioning were evaluated. Using the monobiosensors and the biosensor system analysis of the ATP and glucose concentrations in pharmaceutical samples, and creatine kinase activity in blood serum was carried out. Tests of the biosensor system demonstrated good correlation of the obtained data with the nominal, given by producers, concentrations of these substances. Obtained laboratory prototype of the biosensor system for determination of ATP and glucose can be used for analysis of pharmaceutical and biological samples.