Nikolaienko T. Angiogenic profile of cultured endotheliocytes under conditions of microenvironment modifications by pro- and antiangiogenic factors

Dissertation is devoted to evaluation of angiogenic profile cultured endotheliocytes according functional and metabolic parameters such as proliferation, apoptosis levels, glucose utilization and production of nitric oxide. We is reproduced some models in vitro of vascular endothelial growth due to lack of trophic substrates, partial hypoxia, selective influence of VEGF and anti-VEGF and indirect effects of pro- and antiangiogenic factors as potential therapeutic agents. Revealed that mitokorektin and Calix [4] arenas C-145 have proangiogenic effect on endothelial cells, analogues HIF-1 inhibitors - syn- and anti-isomers of 2- (2-aminotiazolil) hydroxyiminoacetic acid and activated by visible light photoforms peptidomimetics (analogue of gramicidin S) are demonstrated antiangiogenic effects. Reproducible model system of culture endothelial cells under different conditions of microenvironment can be used for screening of substances with angiogenesis-associated mechanism of action and provide perspective of further study of pro- and antiangiogenic therapy.