Chyzh Y. Biotechnologies based on magnetically labeling of microorganisms

The thesis is devoted to the substantiation of natural magnetically labeled of biological objects and the development of biotechnology of artificial magnetically labeled of microorganisms on the model yeast cells Saccharomyces cerevisiae, based on magnetohydrodynamic stirring in crossed electric and magnetic fields. Microorganisms with properties that can be used as vectors to deliver drugs to tumors was detected. Force of magnetic dipol-dipol interaction between chains of magnetic nanoparticles in biological objects and chains of biogenic magnetic nanoparticles in tumor cells was calculated, which is about 10^-9 N and is close to the forces binding antigen-antibody. Technological scheme of receiving magnetically labeled biosorbent was developed. The optimal process parameters was found: pH 2.5; U = 0,5 V; t = 6 min, H = 240kA/m. Experimentally shown that the sorption ability of magnetically labeled biosorbent based on magnetohydrodynamic stirring in crossed electric and magnetic fields, on 30% more than in magnetically labeled biosorbents produced by other methods.