Mutenko H. The genes of afs-, aco- and adpA-families involved in pleiotropic regulation of secondary metabolism in Streptomyces ghanaensis ATCC14672

S. ghanaensis ATCC14672 genome contains a group of structural and regulatory genes for the synthesis of two LMWC of butyrolactone and avenolide classes. The knockout of the acogh gene blocks the production of moenomycins. The afsAgh gene knockout impaired formation of the aerial mycelium on rich media, but had no effect on the synthesis of antibiotics. Inability of acogh mutant to produce MmA was not associated with significant changes in the transcriptional activity of some key moe genes as well as their pleiotropic transcriptional regulators. Influence of the above genes on S. ghanaensis is likely mediated by some pleiotropic regulators, such as that of AdpA family. Indeed, adpAgh mutant of S. ghanaensis did not complement the phenotype defects of afsAgh mutant. Obviously, to stimulate a positive regulatory cascade (in particular, sporulation), S.ghanaensis. We analyzed wild type S. ghanaensis strain, and its aco and moeO5 mutants. The root colonisation of model objects of mono- and dicotyledonous plants (Hordeum vulgare and Lycopersicon esculentum) by streptomycetes was investigated by in-situ fluorescence hybridization and scanning microscopy. Wild type, as well as a number of mutants of S. ghanaensis and S. lividans, colonize the roots of H. vulgare and L.esculentum. It should be noted that streptomycetes, colonized the root hairs better than the root surface directly. In many cases bacterial LMWC are the activators of plant systemic resistance against pathogens. However, it is still not clear how the simplest artificial analog of ?-BL affects on plants.