Hretskyi I. System for express evaluation of biological action of non-ionizing electromagnetic radiation using Photobacterium phosphoreum ІМV В-7071

The constant increasing load on the biosphere from the anthropogenic non-ionizing electromagnetic radiation (EMR) of the radio frequency range has become the global environmental issue. Despite the great interest in the studies of the biological effects of radio emission the results of the research in this area are often very variable, contradictory and require a deep systemic analysis. Traditional technical means that determine the physical characteristics of an electromagnetic field do not allow to assess the biological effect of the EMR. This increases the relevance of the stydies of of the integrated action of radio emission on the example of relatively simple and well-studied model organisms. Among the existing biological test objects, luminescent bacteria are important and suitable for determining the action of various physical and chemical factors in real time. The high sensitivity, simplicity and cost-effectiveness of luminescent test systems make it possible to further expand of their application to assess the environmental risks. Therefore, today it is relevant to create an easy, fast and cheap method for determining the biological effect of EMR using luminescent bacteria. The purpose of this work was to develop scientific and technological approaches to the creation of a biotesting system for detection of electromagnetic radiation of the radio frequency range with the participation of luminescent bacteria. We obtained results regarding the sensitivity of luminescent bacteria Photobacterium phosphoreum IMV B-7071 to the action of EMR in a wide range of power and frequency. The changes in bacterial luminescence of P. phosphoreum IMV B-7071 on different densities of media was investigated. The scheme of a test system for assessing the biological effect of EMR on the basis of luminescent bacteria P. phosphoreum IMV B-7071 was developed and its analytical characteristics were determined. The biotesting of the EMR Wi-Fi router using the developed test system showed a irradiation negative impact on bacterial luminescence. The procedure for determining the influence of perturbations of a geomagnetic field on bioobjects with the help of a constructed test-system was developed. It was proposed the estimations scale of biological action of EMR according to ecotoxicological monitoring standards.