Babkina M. Biotechnological ground for designing of bactericidal activity preparations on the basis of modified polyacceptor compounds.

The thesis is devoted to the determination of antibacterial activity of novelsynthesized modified heterocyclic compounds and determination of their minimal inhibitory concentration. Experimental work was carried out using the selection of cell cultures, microorganisms, optimal solvent for the investigated compounds. The microorganism cultures and cell cultures were used for research. In a number of studies it was proved that the best solvent for novelsynthesed modified heterocyclic compounds was dimethylsulfoxide (DMSO), because it showed low toxicity and ability for complete dissolution of the compounds and conservant properties for the solution. Minimum inhibitory concentration of substances was determined. Compounds of different classes have shown different antibacterial activity in relation to the tested microorganisms. It was selected 6 substances according to the determination of minimum inhibitory concentration (MIC), which had antimicrobial activity for all 8 test microorganisms. It was selected 6 substances, which were tested for cytotoxicity in per-massive cell lines. It has been determined that these substances did not reveal any toxic effects on the monolayer of per-massive HeLa, Vero and BHK cell lines. It was proposed the new method for antimicrobial activity detection.