Puhovkin A. Osmotic response of freshwater fish spermatozoa to the action of cryopreservation factors

The resarch object was the cryopreservation of freshwater fish sperm. The research aim was to establish the relationship between the osmotic response of freshwater fish spermatozoa to the action of cryopreservation factors and their cryoresistance. Research methods were EPR spectroscopy to determine the change in the content of free intracellular water in freshwater fish spermatozoa; spectrophotometry for study of the osmotic response and determination of the concentration of freshwater fish spermatozoa; physical and mathematical modeling for calculation of plasma membranes permeability coefficients of freshwater fish spermatozoa to water and cryoprotectant molecules; light microscopy to determine the parameters of the freshwater fish spermatozoa motility; hormonal stimulation of maturation to produce sperm; cryopreservation of freshwater fish spermatozoa, statistical analysis. The method of determining the permeability of the freshwater fish spermatozoa membranes to water molecules has been designed. For the first time the coefficients of membrane permeability of carp, sterlet and pike spermatozoa to water molecules, and the activation energy of water transfer through the spermatozoa membranes, was determined. For the first time the coefficients of membrane permeability of carp spermatozoa to dimethylsulfoxide, ethyleneglycol, 1,2-propanediol and glycerol cryoprotectants, and the activation energy of substance transfer through the spermatozoa membranes,were determined.