Pokhylko S. Technological aspects of bread wheat biofortification of Gpc-B1 gene from Triticum turgidum ssp. dicoccoides

The dissertation is devoted to the biotechnological aspects of winter bread wheat biofortification by the Gpc-B1 gene introgressed from Triticum turgidum ssp. dicoccoides, which increases the content of protein and minerals in grain. Molecular-genetic marker systems for the complex analysis and selection of bread wheat hybrids, the carriers of Gpc-B1 gene from Triticum turgidum ssp dicoccoides, have been developed. The 44 lines of F5 generation, homozygous in target gene were selected from 160 plants of F2 generation. The generation of F5 hybrid lines was thoroughly characterized by the puroindolins and glutenins genes. The macro- and micronutrient content analysis was performed. The content of total protein in grain was determined, and eventually, 13 hybrid lines were selected for the future work. The analysis of yield and physiological parameters of lines-carriers of Gpc B1 gene from Triticum turgidum ssp. dicoccoides showed that the lines were resilient to lodging, medium-growing with medium yields. Key words: Triticum aestivum, gene Gpc-B1 from Triticum turgidum ssp. dicoccoides, biofortification, DNA markers, high content of protein, macro- and microelements.