Negrych N. Clinically-pathogeneticand dsagnosticvalue of protein nature serum markes in patients with multiple sclerosis

The dissertation is devoted to the discovery and study of clinical and pathogenetic features of the course of multiple sclerosis (MS) and the study of new serum biomarkers of protein nature in patients with the aim of improving diagnostics and prediction of the course of this disease. By using immunological techniques in the serum of patients with MS was first discovered and identified 48 kDa form of unconventional myosin 1c (48 kDa Myo 1c) and serine-proline-cysteine ??oligopeptide (Ser-Pro-Cys peptide) and allocated hiperhlikozylovani immunoglobulins (Ig ) It was established that the substances isolated from the blood serum of patients had marker properties in the PC. In particular, the level of 48 kD Myo 1c in serum of patients was statistically significantly higher than that of the control group, and the increase in this protein indicates an increase in the processes of apoptosis of activated B-lymphocytes and is associated with a more benign course of the disease. It has been shown that Ser-Pro-Cys peptide concentration in serum of patients with MS is statistically significantly higher than that of the control group and reflects the activity of the disease. It is confirmed that the appearance of hyperglycosylated immunoglobulins in blood serum of patients with MS is associated with exacerbations of the pathological process, indicating the active participation of the studied modified Ig in the development of active demyelinization in this disease. The algorithm of the use of laboratory biomarkers at RS is developed and structured, which includes application of laboratory tests for diagnostics of MS, determination of its phenotypic manifestations and characteristics of the effectiveness and safety of therapy.