Gramatyuk S. Pathogenetical patterns of development of endogenous intoxication syndrome with hepatic encephalopathy in the course of chronic hepatitis C

The thesis gives theoretic generalization and a new solution of the scientific problem, which is to establish pathogenetical patterns of endogenous intoxication syndrome with hepatic encephalopathy in the course of chronic hepatitis C. The study was divided into two stages: on the first – an examination of patients with chronic viral hepatitis C was performed; In the second stage ? an in vitro experimental study in human hepatocyte mitochondria was infected with hepatitis C virus, the control group was hepatocytes that were not infected with HCV infection. The study results of immune and metabolic processes in chronic course of hepatitis C with symptoms of hepatic encephalopathy have been established. It is proved that endogenous intoxication is always inherent in the development of chronic hepatitis C and, in turn, has a significant impact on the further course of the disease. Our results of glyceraldehyde-3-phosphate dehydrogenase activity confirms the assumption of an increase in the energy needs of the cell. Hepatitis C virus induces mitochondrial deformation and causes oxidative stress and increases the intracellular calcium content, which causes dysfunction and damage to mitochondria. Disorders of the mitochondrial membrane is a consequence of HCV infection and is not selectively induced in cells with a high replication rate. The results suggest that HCV infection induces mitochondrial deformation. At the same time, it has been shown that in the development of endogenous intoxication a significant contribution is made by disorders of cellular metabolism in the occurrence of which an important role is played by virus-induced disturbances in mitochondrial energic potential.