Boychuk Y. Selection and in vitro culture establishment of highly productive genotypes of spring false flax (Cimelina sativa L.) for their further genetic transformation

The study presents the results of the comparative analysis of the morpho-physiological and biochemical characteristics of new variety samples and varieties of false flax (Camelina sativa) of Ukrainian breeding, and selected the most promising ones. Among eightvariety samplesandfour varietiestherewere found the highly productive genotypes, two varieties and one variety sample which, due to their specific morphometric and their biochemical properties, are very important. Conditions for establishment of in vitro culture and shoot regeneration of C. sativa have been found. In particular, the age and type of explants for efficient plant regeneration have been determined. The influence of phytohormones on shootinduction and root development of C. sativa has been investigated. The analysis of regenerative ability of all investigated false flax genotypes was carried out. A genetic transformation was carried out using two methods and it was demonstrated that Agrobacterium-mediated transformation in planta is the most effective method for foreign genetransfer to C. sativa genome. The transgenic nature of selected plants was confirmed by molecular genetic analysis using specific primers to GUS gene.