Shovkova O. Development and validation of the methods of secnidazole determination for chemical-toxicological analysis

The thesis is devoted to complex chemical-toxicological analysis of biological liquids for secnidazole. The behaviour of secnidazole under systematic toxicological TLC-analysis in combination with colour reactions on the plates has been investigated. The procedures for detection and quantitative determination of secnidazole by the methods of spectrophotometry, HPLC/UV, HPLC/MS, GLC/FID and GLC/MS have been developed, their validation in the variants of MCC, MS and MA has been carried out using model solutions. Acid-base equilibria of secnidazole in aqueous solutions and mixtures of water with amphiphilic solvents (1:1) have been studied and efficiency of secridazole extraction from aqueous solutions in two directions has been investigated – liquid-liquid extraction with organic solvents immiscible with water and liquid extraction by amphiphilic solvents. The procedures of sample preparation of biological liquids for their analysis for secnidazole have been proposed, their validation in the variants of MCC, MS and MA has been carried out; the total uncertainty of the procedures has been calculated.