The object of the study is the course of dyscirculatory encephalopathy (DE) in postmenopausal period with the implantation of cryopreserved placental explants (CPE). The aim of the work was to study the effect of cryopreserved placental explants on the course of dyscirculatory encephalopathy in postmenopausal period in experimental models. Methods were as follows the ones of cryobiology, cryomedicine and experimental medicine (excitotoxicity modeling, postmenopause (PMP), DE, implantation of CPE); cultural methods (cultivation of neural cells (NC) of experimental animals (EA)); study of the general physical characteristics of EA; neurophysiological methods: study of the behavioral reactions of EA, evaluation of interhemispheric asymmetry, cognitive-mnestic functions of EA; histological and morphometric studies of brain tissue of EA; statistical analysis of the data. Theoretical and practical results, scientific novelty: it has been shown that the media, conditioned with CPE have neurotrophic and neuroprotective effects in glutamate-induced exitotoxicity model on NC in vitro. It is proved that CPE implantation does not change the lifespan of female mice, but reduces the probability of their death in the reproductive period, has positive effect on their zoosocial, orientational, research activity and significantly reduces anxiety. The average lifespan of male mice after CPE implantation increases by 18%, the maximal enhances by 17%, the general appearance and physical strength are improving, however indexes of orientational, research, zoosocial and anxiolytic behavior are deteriorating. During studying the effect of CPE implantation on neurophysiological indices of ovariectomized mice with DE model the improvement of cognitive functions and central nervous system adaptation capabilities were determined. It was found that CPE implantation leads to the restoration of microcirculation, cytoarchitectonics and stimulation of proliferation of endotheliocytes and pyramidal neurons of the outer layer of the motor cortex and that of cornus Ammonis dentate gyrus of the hippocampus. CPE implantation has a positive effect on the course of experimental comorbid pathology, and can be considered as a promising, pathogenetically validated method for overcoming DE in women with postmenopause and its consequences. Practical significance: the defined CPE neuroprotective effect on NC in vitro justifies the use of media conditioned with CPE for effective restoration of NC cultures in the protocols for their cryopreservation and low-temperature storage. The positive effect of CPE implantation on the physical indices, life expectancy, behavior, stress resistance and cognitive-mnestic functions of mice in the DE model in PMP with the restoration of microcirculation and cytoarchitectonics in the hippocampus and motor cortex has been shown to prove the promise of CPE application for the correction of cerebrovascular geriatric changes and improvement of methods of DE therapy in PMP. The results of the study made it possible to pathogenetically substantiate and develop a “Method for the treatment of cognitive impairment in postmenopausal dyscirculatory encephalopathy” (Patent of Ukraine No. 124910). Field of use: in cryobiology, cryomedicine, in educational programs and scientific research, in gerontology, gynecology and neurology.
