Oliinyk O. Optimization of Biotechnological Process of Microclonal Propagation for Rose Essential Oil (Rosa Damascena Mill.).

The biotechnological process of planting material obtaining for highyielding varieties of rose essential oil cultivation has been optimized with the study of the morphogenetic, physiological and anatomical potential of their tissues and organs in vitro. The research materials were the model varieties Lan’, Lada and Raduga of Rosa damascena Mill. It has been shown that the activation of polyphenol synthesis occurs responding to traumatic damage of primary explants tissues under conditions of plant introduction into in vitro culture.The conditions of rhizogenesis, soil mixtures and substrates for the step-by-step procedure of stepwise adaptation of regenerated plants for the open ground conditions with a survival rate of 95–98 % for species of Rosa damascena Mill. Lan’, Lada and Raduga were selected. According to the results of propagation, we have developed a mathematical model of cyclic biotechnological process of Rosa damascena Mill. microclonal propagation, which is based on the basic physiological characteristics of model varieties and allows to optimize the technology of continuous production of quality planting material for a long time.