Velichko N. Long-term effects of neonatal influence of phytoestrogens and stress on the reproductive function and hormonal-metabolic parameters in adult males of rats (experimental research).

The work is devoted to the study of delayed effects of emotional stress and excess phytoestrogenization during breastfeeding on the reproductive function of male rats in adulthood, the state of the testes, the content of sex hormones, some metabolic aspects of protein, lipid and lipid. Object of the study: violation of the functional activity of the sexual glands of male rats, hormonal-metabolic processes and reproductive function due to neonatal interventions (emotional stress, excess phytoestrogens, combined influence of factors). Aim of the study: To determine the peculiarities of the functioning of the reproductive system and hormonal-metabolic state of adult male rats who were exposed to emotional stress in the neonatal period, the admission of excess mixture of phytoestrogens or the combined effect of these factors. Methods: physiological, ethological, biochemical, cytological, histological, immuno-enzymatic, statistical. It is experimentally proved that the effect of applied factors has imprinting consequences, which changes the course of biochemical processes and adversely affects the state of the reproductive system in animals of the studied groups. This is manifested in the violation of spermatogenesis, decreased sexual activity, reproductive potential and is accompanied by imbalance of sex hormones, reduction of physiological sex differences of some metabolic aspects of protein, lipid metabolism with the development of chronic oxidative (nitrosyde) stress. Under conditions of combined influence of stress and excessive phytoestrogenization during the period of breastfeeding in males, the adult develops a state of latent hormonal imbalance, which cannot be determined in the study of hormones in serum alone. Against the backdrop of slight multidirectional changes in sexual behavior and sperm count, the reproductive potential is more than halved, that is, the potential number of offspring in these animals. This condition can be considered a pathogenetic model of neonatal-induced male hypo-fertility of uncertain genesis with metabolic disorders. As a result of the work, a method for modeling neonatal-induced hypo-fertility of male rats with metabolic disorders was developed and put into practice (Patent of Ukraine for utility model № 95758) and actualized in the educational process – «Institution of Endocrinology and Metabolism named V. Р. Komisarenko of NAMS of Ukraine», Kyiv Bogomolets National Medical University.