Cherniavska O. Effect of rhythmic extreme cryoexposures and cord blood on the state of regulatory systems of rat body in alimentary obesity.

The research object was the structural and functional state of regulatory systems of animals of different age groups in a normal state, that with alimentary obesity (AO), the one after rhythmic extreme cold effects influences (RECE) (–120°C), as well as after the introduction of cryopreserved cod blood cells and during their combined use. The research aim was to determine the influence of RECE (–120°C), human cCBNCs and their combination on structural and functional state of regulatory systems of animals of different age groups with the modeled AO. Research methods were as follows: cryobiological (RECE, –120°C); electrophysiological (recording of electrocardiogram with subsequent spectral analysis of heart rate variability to assess the state of adaptation reserves and systems of neurohumoral regulation of an animal body); immuno-enzymatic and biochemical methods for determining the concentration of thyroid (thyroxine, triiodothyronine) and sex (testosterone, estradiol, dehydroepiandrosterone-sulfate) hormones, as well as the study of lipid profile of serum, lipid cholesterol, total cholesterol, low density, coefficient of atherogenicity); histological, histochemical and electron microscopic methods for investigation of the structure of the myocardium tissues and vessels as one of the major markers that reflects the AO development; AO modeling technique; statistical methods of experimental data analysis. For the first time, the structural and functional changes that occur in regulatory systems of animals’ body of different age groups after RECE, CBNCs and their combination in the norm and on the background of the AO development have been assessed. For the first time, it has been established that the combined use of RECE and CBNCs is able to maintain the "young" rhythm of neurohumoral effects on a body, to promote the implementation of hormonal regulation, normalization of serum lipid profile and reduction of body weight, activate metabolic and synthetic processes at microscopic and submicroscopic levels.