Shatskyi V. Functional and morphological renal impairments under conditions of acute blood loss, complicated by limb ischemia-reperfusion, and their correction

The role of the limb ischemia-reperfusion, caused by two-hour tourniquet application to the femur, in the pathogenesis of renal prooxidant, functional and morphological impairments in the presence of the acute blood loss of 20-22% of circulating blood and the substantiation of corrective properties of the carbacetamin reducing manifestations of identified impairments has been established in the thesis work. In conditions of acute blood loss complicated by limb ischemia-reperfusion, the aggravation of renal prooxidant, functional and morphological impairments has been revealed compared to only acute blood loss model. A significantly higher thiobarbituric acid reactive substances content as well as lower catalase activity, significant decrease in the diuresis , in the proximal and distal transport of potassium ions and in the sodium-free water clearance occur in the renal cortex and the renal medulla at all observation periods. The significantly lower glomerular filtration rate is defined after 4 hours and 7 days of the reperfusion period. It has been determined that a concentration of sodium and potassium ions in the urine and their excretion increase from the first day of the experiment. Morphologically, destructive changes of epithelial cells in the tubules accompanied by basement membrane damage are observed, which augment up to 7th day and marginally decrease up to 14th day. It has been established that carbacetam administration to rats with acute blood loss complicated by ischemia-reperfusion, compared to animals without treatment, contributes to the enhancement of prooxidant mechanisms and improvement in morphological and functional conditions of the kidney after 7 days. The efficacy of the carbacetam intensifies after its administration for 14 days. Despite the fact that studied parameters do not reach the control level, the carbacetam is considered to be promising pathogenetic treatment for correction to the studied pathology.