Semionova K. Mammalian erythrocyte sensitivity to cooling, dehydration and rehydration under glycerol and modifiers effect

The research object was the posthypertonic shock of mammalian erythrocytes. The purpose of this study was to determine the posthypertonic hemolysis of mammalian erythrocytes (human, rat, pig and rabbit) and glucose effect on ones value, and also to estimate chlorpromazine efficacy in the conditions of posthypertonic shock and at the stage of glycerol removal from thawed erythrocytes. Research methods were as follows: cryobiological, microhematocrit, ionometric, osmometric, spectrophotometric, method of erythrocyte mechanical destruction, light thermostat microscopy and statistical analysis of the obtained data. For the first time, erythrocyte posthypertonic hemolysis of the studied mammalian species depends on both the experimental conditions and the cell types. The dehydration medium osmolality and the duration of cell incubation in it determine the level of mammalian erythrocyte posthypertonic lysis. Protective effect of glucose and chlorpromazine on erythrocytes during posthypertonic shock at 0°C has been first shown. Chlorpromazine reduces the human erythrocyte hemolysis during cryoprotectant removal from thawed erythrocytes after their freezing down to -196ºC under glycerol protection.