The object of research: intestinal microbiome, salmonella, GALT. The purpose of research: to elucidate the patterns of salmonella-induced changes in the intestinal microbiome and the transcription of the FFAR 2, Foxp 3 and RORγt genes of the rat immune response on the background of vancomycin administration and in the conditions of B. fragilis correction. Research methods: Bacteriological (isolation of pure cultures of microorganisms, their identification and determination of sensitivity to antibiotics), microscopic (determination of microorganisms by their morphological and tinctorial features), pathophysiological (modeling of salmonella-induced inflammation (SIII)), morphometric, platelet determination (identification of immunopositive cells), chromato-mass spectrometric methods of analysis (determination of SCFA concentration), molecular genetic methods (quantification of microorganisms, detection of antibiotic resistance genes and assessment of relative mRNA levels by real-time polymerase chain reaction (PCR-RT)), computer image analysis and mathematical classification analysis (calculation of the number of immunopositive cells), methods of statistical analysis of the results. Equipment: PrimoStar microscope (ZEISS, Germany); AxioCam 5c camera (ZEISS, Germany); liquid chromatography-mass spectrometer: Agilent 1260 Infinity HPLC System (USA); amplifier and program CFX96 ™ Real-Time PCR Detection Systems (Bio-Rad, USA); computer program ImageJ (NIH, USA). The thesis is devoted to the development of treatment of salmonella-induced changes in the intestinal microbiome and transcription of FFAR 2, Foxp 3 and RORγt immune response genes. Changes in the composition of the intestinal microbiota of rats with the introduction of vancomycin, S. enteritidis, S. typhimurium and B. fragilis were analyzed. The ability of bacteroids to reduce the manifestation of SIII by increasing the number of Bacteroides spp., E. faecalis, E. faecium, Lactobacillus spp. and reducing Salmonella spp., P. aeruginosa, Enterobacter spp., Klebsiella spp., E. coli. Genes of resistance to carbapenems, cephalosporins and glycopeptide antibiotics have been identified in microorganisms of the families Enterobacteriaceae, Bacteroidaceae, Peptostreptococcaceae, Enterococcaceae and P. aeruginosa. The effect of immunoregulatory bacteria, B. fragilis on gene transcriptional activity and SCFA concentration has been experimentally proven. The effect of vancomycin, salmonella and B. fragilis on the level of expression of effector proteins of salmonella Sop B, SopE 2, Sip A and on the number of Sytox + cells and NETs with SIII was established. Theoretical generalization and solution of scientific and practical problem is the possibility of using the culture of B. fragilis to correct pathological changes in the intestinal microbiome with salmonella-induced infection. It is recommended to use cultures of B. fragilis as a possible adjuvant therapy in combination with antibacterial drugs in the treatment of SIII. The extent of implementation: 7 acts of implementation, 1 patents. The field (industry) of use: healthcare (medicine: microbiology, immunology, infectious diseases).
