Pallah O. Formation of bacterial biofilms under the influence of biologically active substances of plant origin

The dissertation is devoted to the detection of: 1) ability of opportunistic bacteria isolated from surfaces of edible plants and medical equipment to form biofilms 2) spectrum (quantitative and qualitative content of polyphenols and anthocyanins in berries of edible plants (blueberries, plums, yostа, cherries, plums, black and red currants) 3) ability of extracted water solutions of polyphenols and anthocyanins from berries to inhibit biofilms formed by clinical and plant bacterial isolates. It was found that the bacterial strains isolated from hospital surfaces are characterized by multiple resistance to modern antibiotics, and are more resistant to their action compared to bacterial strains that have been isolated from plant surfaces. Significant difference in the strength of attachment to the biotic surface between the bacterial strains isolated from hospital surfaces and bacterial strains isolated from the plant surface was shown. The difference in the formation of biofilms on abiotic surface by clinical and plant surface bacterial isolates compared to commensal strains was established. The highest inhibitory effect on the growth of the bacterial strains isolated from hospital surfaces and bacterial strains from the surface of edible plants demonstrated biologically active compounds from plums, plums, cherries and black currants. The ability of plum extract to stimulate the growth of strains of L. acidophilus C-01, L. acidophilus C-03 was established. The most significant influence on the biofilms of the bacterial strains isolated from hospital surfaces and bacterial strains isolated from the surface of edible plants was caused by plum extracts. It has been established that biologically active substances such as anthocyanins, namely cyanidin-3-rutinoside, have probacterial properties against lactobacilli. Plum extract at a concentration of 0.002 mg/ml did not demonstrate toxic effect on the culture of dermal fibroblasts in newborns. A scheme for designing new biological products based on plum extract and author's strains of lactobacilli has been developed to alleviate caries, periodontal inflammation, obesity and type 2 diabetes.