Mazurenko V. Comprehensive biotechnological diagnosis of contagious mastitis in cows

Biotechnological process of the food industry craves to provide the market with quality and valuable food that can only be obtained by using high-quality milk.As for the milk quality, it is influenced by the following indicators: the number of somatic cells, the absence of pathogenic bacteria and inhibitors (residues of veterinary drugs, antibiotics, hormones, etc.), exogenous contamination.In this regard, the search for new measures to increase milk productivity and improve milk quality is relevant and has scientific and practical significance. The thesis is devoted to the identification of bacterial isolates taken from Ukrainian milk cows. The author analyzed the antibiotic sensitivity and antibiotic resistance of bacteria. For the first time in Ukraine, the use of lactate dehydrogenase for the initial detection of early subclinical infection has been proposed. The assessment of the spreading of bacterial isolates on farms in Ukraine suggests that the prevalence of pathogens is growing and this issue needs to be addressed. The study has shown that in case of subclinical infection of mammary gland 100 % of selected isolates are contagious mastitis pathogens. However, due to analyzed mastitis samples (clinical and subclinical forms) from different farms of Ukraine, it was found that 15 % of samples were positive for Staphylococcus aureus and 26 % of samples – for Streptococcus agalactiae, 59 % of samples contained non-contagious pathogens of mastitis. The novelty of the work is as follows for the first time, a scientific approach to monitoring udder health status of cows for subclinical inflammation has been improved. It is established that as a result of control of contagious pathogens on the farm the quality of cow's milk will improve. A determination of the enzyme lactate dehydrogenase as biomarkers of inflammation of the udder and reasonably complex combination of biological research methods for the determination of infectious agents in secret udder of cows.