Romak O. Pathogenetic mechanisms of liver tissue regeneration under conditions of correction of experimentally induced chronic hepatitis and liver cirrhosis

In dissertation the mechanisms of morpho-functional state of the liver in the conditions of modeling of chronic hepatitis (CH) and liver cirrhosis (LC), as well as the effectiveness of correction of the detected changes by using platelet-rich plasma (PRP) were studied. In accordance with the purpose and objectives of the study, the experiment was performed on 205 adult male rats, which were kept in standard conditions of the biological clinic (vivarium) of Odessa National Medical University. Using pathophysiological (experimental modeling of СH, LC; PRP introduction), pathohistological, cultural (isolation of PRP from whole blood), hematological (hemogramma), biochemical, immunoenzyme (proinflammatory cytokines) and statistical methods revealed the dynamics of morphological changes, functional state of the liver under the conditions of administration of PRP, in modeling the pathology and after its correction. CH was induced by the oral administration of an oil solution of tetrachloride carbohydrate (CCl4) at a concentration of 50 % in a dose of 0.05 ml per day for 7-8 weeks. LC was induced by oral administration of an oil solution of CCl4 at a concentration of 50 % in a dose of 0.05 ml per day and water substitution by ethil alcohol at a concentration of 10 % for 150 days. PRP was obtained by isolating it from whole blood by centrifugation with sodium citrate on a SmartPrep apparatus (Harvester Corp., USA). All results were processed using conventional and biomedical studies of parametric and nonparametric methods of statistical analysis. The 1st day of the experiment was considered the day after the last injection of CCl4 or PRP.