Lashuk S. Creation of initial selection materials of representatives of the genus Miscanthus by the method of regulation of their reproductive development and the use of biotechnological methods

The dissertation presents the theoretical justification and methodical development of new modern biotechnological methods of miscanthus propagation and the creation of new initial forms to increase the genetic diversity of existing species from the point of view of their use as raw materials for bioenergy. The methodical foundations of miscanthus biotechnology have been improved, in particular, schemes and modes of sterilization of seeds and buds with rhizomes, prescriptions of nutrient media for seed germination and microclonal propagation of miscanthus in vitro. A method of obtaining callus lines of M. sinensis and M. sacchariflorus in vitro using initiation of callusogenesis and regeneration of microplants from callus has been developed, which makes it possible to increase the multiplication factor of microplants of M. sacchariflorus by an average of 40 times, M. sinensis − by 20 times, comparing with foreign counterparts. A method of adaptation of miscanthus regenerants in open ground has been developed, which involves stimulating the growth of rhizomes with the use of nutrient media prescriptions, which included gibberellin as the main phytohormone. The use of this phytohormone helps to increase the length of rhizomes on nutrient media and ensures the guaranteed preservation of micro-plants propagated from in vitro culture during adaptation in open ground without the use of greenhouse complexes.