Sysa I. Studies on peculiarities of transition metal ions interaction with nucleic acids of different levels of structural organization

The objects of study: NA metal complexes in water solutions. The aim of the study is to determine the nature of differences in transition metal ions binding to NA, to find out the reasons of different influences of the metals on DNA conformational state and to reveal the influence of different levels of NA structural organization on the complex formation. Methods and equipment are differential UV spectroscopy, thermal denaturation. Results, novelty: heteroatoms of NA bases, coordinating ions, were determined and corresponding binding constants were calculated. It was found that the differences in the ions complex formation with DNA are determined by differences in cytosine binding showing already in monomeric level. For the first time the formation of two different silver complexes has been registered by the method of thermal denaturation. An alternative model of a weak complex is proposed. The fields of application are molecular biophysics, ecology.