Lupilova N. The influence of anion transport inhibitors and cytoskeleton modificators on barrier characteristics of erithrocytes under the osmotic stress

The subject of the investigation is the donor human RBC. The aim of the investigation is to study the RBC barrier characteristics of erythrocytes with normal and modified cytoskeleton under media factors witch consists of electrolytes and nonelectrolytes. The main methods are spectrophotometry (spectrophotometer-4A), Spectrofluorimetry (Fluorescence spectrophotometer MPF-2A "HITACHI")^ pH-metry (ionometer universal-74). The scientific novelty is that the mechanisms of RBC anion channel functioning in the hipertonic conditions under the effect of anion inhibitors (DIDS, dipyridamole) and modificator of cytosskeleton (hemin) have studied for the first time. The obtaind results have shown that the exgange transport chloride on sulphate is more sensitive to hipertonic and temperature influence and cytoskeletal modificators effects than pure efflux chloride; the anion transport inhibitors block effect is determined both by the inhibitor nature and the media conditions in witch cells get into during the cool ing. The results of the work allow to based theoretically new approaches to the devise methods of RBC lowtemperature preservation in response to the influence of temperature and osmolarity of the solution of preservation. The practical significance is that the results can be used in hematological and cryobiological practice as allow to give recomendations conserning the methods of the cell state regulation and useing the devised methods the analysis of RBC anion channel functional state for diagnostics.