Berest V. Influence of temperature on platelet aggregation under normal conditions and under action of gamma-irradiation

Fibrinogen, human and rat platelets. The study of molecular mechanisms of temperature and gamma-irradiation effects on platelet aggregation. VHF-dielectric spectroscopy, UV-spectroscopy, lightscattering. The fibrinogen conformational transition and structural changes of platelet membranes have been detected at 18-20, 30-32oC. The temperature dependences of the aggregation parameters have been obtained for various agonists. The mathematical model of platelet aggregation have been built. The values of rate constants and activation energy have been determined. The effect of gamma-irradiation on platelet aggregation was studied both in vitro (10-250 Gy) and in vivo (0,16-50 cGy). Application field - cell biophysics, radiation biophysics.