Gregirchak N. Development and inculcation of recipiency method neuston biopreparations by means of flotation.

The subject of the dissertation is the development of manufacturing and application biotechnologies of neustonic biopreparation. Neustonic biopreperation іs based on the cells with increased hydrophobicity. This character allows them to concentrate within the surrface layer of water and to intensify its purification. New flotative method for the selection of the cells with high hydrophobicity was developed. Flotation allows to select the cell fractions, which differ by their morphological and biochemical properties. One neustonic biopreparation from Bacillus spp. with wide spectrum of antimicrobial activity was proposed to use for the decontamination of surface layer of water. Another biopreparation , which is based on neustonic variant of Bac. megaterium 1BD, was proposed for the bioremediation of the oil polluted water. Application of this biopreparation in the field showed that it significantly increased the bioremediation of water polluted by oils and decreased the sum the damage to envir onmental in 40 times. Key words: neuston, bacterial preparation, flotation of microorganisms, hydrophobicity, flow cytofluorometry, synchronization.