Object of the research: Mechanisms of isolated and combined action of ionizing radiation and sodium fluoride on the organism. Purpose of the research: To determine the state of oxidation metabolism at isolated and combined action of ionizing radiation and sodium fluoride as well as capabilities of preventive and therapeutic correction with antioxidants. Methods of the research: Pathophysiological, toxicological, biochemical, radiobiological, statistical. Theoretical and practical significance of the obtained findings: The obtained findings are of both theoretical and practical significance. They can be used for theoretical substantiation of AO application in the clinical practice at radiation exposure and fluoride intoxication. AO complex, which considerably corrects the disorders, occurring at exposure to ionizing radiation and sodium fluoride was suggested. It was shown that it increases survival in animals by 36-40%. Optimum terms of the use of AO complex at exposure to ionizing radiation and chronic action of sodium fluoride on the organism were revealed. Novelty: Complex evaluation of the state of oxidation metabolism at isolated and combined action of two ecologically unfavorable factors (ionizing radiation and sodium fluoride) was done for the first time. Their effect on LP, AOP system as well as energy metabolism (oxide phosphorylation, activity of adenylate cyclase system, synthesis of RNA, nuclear histones, non-histone proteins) was investigated at various doses, including low ones. It was established that changes in LP, AOP in the liver and blood depended on the doses of radiation, sodium fluoride and their combined action. Low-dose ionizing radiation and sodium fluoride stimulate AOP in the tissues and caused LP products accumulation in the blood; high doses inhibited AOP and caused accumulation of LP products in the blood, especially in the tissues. It was revealed that ionizing radiation and sodium fluoride considerably reduced ATP concentration, and, respectively, energy potential. A definite dependence in the dynamics of ATP, cAMP, and cGMP was observed. Combined action of ionizing radiation and 6-month administration of sodium fluoride inhibited plastic processes (synthesis of RNA and proteins, ie, globulins and histones). The findings suggest preventive and therapeutic effect of a definite AO complex (beta-carotene, alpha-tocopherol, ascorutin, unithiol) at isolated and combined action of ionizing radiation and sodium fluoride. The AO complex prevented or corrected profound impairments in LP processes, energy metabolism and thus reduced intoxication development and increased the organism resistance). Degree of introduction: The obtained findings were introduced to the teaching process at departments of pathological physiology, general hygiene and ecology, extreme and military medicine, clinical pharmacology, medical radiology of Kharkiv State Medical University and Ukrainian Dental Academy. A method of prevention of the effect of ionizing radiation (Patent of Ukraine No. 10765A) and method ofprevention and treatment of fluoride intoxication (Patent of Ukraine No. 10766A) were suggested. Sphere (area) of application: medicine, pathological physiology, physiology, biochemistry, clinical medicine, radiobiology.
