Brovarskyi V. The Substantiation of fertile Queen Reproduction Technology

3. Object - fertile queen, sexual system of fertile queen, drone, working bees, semen drone, equipment for artificial insemination of fertile queen. Purpose -to prove theoretically and to develop line technology of reproduction fertile queen at artificial insemination. Methods - zootechnical, morphological, histological, biochemical and statistical. Novelty - scientific rules of bees' poliandry, which are fixed in a basis of the scientific concept of the developed line technology of reproduction fertile queen at artificial insemination, are extended and complemented. Presence of two vaginalis branches of the valve at fertile queens was installed. Its role in regulation and in lying of diploid and haploid egg also was investigated. This enabled to offer a new way of artificial insemination of bee queens by introduction of drones' semen in a queen' vaginal zone. For the first time optimal dozes of drone semen was experimentally determined. It was established a repetition factor and duration of its introduction in the sting chamber of the queen. The new data on semination eggs of fertile queen during her reproductive activity were received. Accommodation and use of portions of drone's semen in sexual ways of the qeen at different ways of its introduction were investigated. It was found out the influence of poliandry on fatherly attributes of female individuals of bee colony. It was established the stimulating influence of drone semen migration and its biologically active compounds on development of ovary and reproductive function of fertile queen. The expediency of illumination application of a the cell under larve carring was considred as reasonable. The influence of the qeen cell corner inclination, ways of qeen cells accommodation in nurse colonies, behaviour of fertile queens and working bees on an output of purebred queens is found out. It enables to improve the technology of their reproduction. Results of researches - are being used in theory, practice and for the study purposes. Branch - animal production.