Evstigneev M. Intermolecular interactions of biologically active aromatic compounds and DNA in aqueous solution

Object of investigation are intermolecular interactions involving aromatic biologically active molecules (BAC) and DNA in aqueous solution. The aim of this work is to get understanding of underlying principles of interaction of aromatic BACs which are differed in their structural (DNA mono- and bis-intercalators) and medico-biological (antibiotics, mutagens, vitamins) properties in aqueous-salt solution and to get insight into the mechanisms of simultaneous (competitive) binding of such compounds with DNA. Methods of investigation: one- and two-dimensional (2D-TOCSY, 2D-ROESY) homonuclear 1H-NMR spectroscopy (500 MHz). In the dissertation work a solution of important problem of molecular biophysics was given for the first time: an interpretation of fundamental mechanisms of regulation of medico-biological activity was made when different combinations of aromatic BAC are used simultaneously and a complete methodology of structural and and thermodynamic study of intermolecular interactions of various groups of BAC in multicomponent solution in presence of DNA was created. Theoretical models and methods developed in this work can be used for analysis and interpretation of NMR experimental data on non-covalent association of any type of aromatic compounds in aqueous solution. Field of application - molecular biophysics, biotechnology, molecular biology and pharmacology.