Object of the research: The function, structure and metabolism of the brain at exposure to VHF radiation. Purpose of the research: To reveal the mechanisms of interaction of the nervous system with VHF electromagnetic filed (2375 MHz), to determine the dependence of the health on the frequency and duration of this exposure based on pathophysiological, metabolic, pathomorphological changes in the brain and nervous system at various level of organization; to analyze the revealed changes and to create the concept of biological action of VHF radiation on the human and animal organism. Methods of research: Clinical-physiological methods (observation of the animals' behavior, method of conditional reflexes, EEG, clinical analysis), morphofunctional diagnosis using light and electron microscopy in combination with the methods of histochemistry and biochemistry, statistical analysis. Practical significance of the obtained findings: The revealed mechanisms of pathologic effect of VHF field can be used when developing protection against microwave radiation, rehabilitation of the persons exposed to high doses of VHF field. The created and tested new experimental model of simultaneous registration of activity of different structures of the brain and peripheral nerve can promote more profound investigation of the mechanisms of the brain activity in various conditions of the life. The obtained findings can be a theoretical basis for further experimental investigations at the study of interaction of organs and systems with VHF radiation. Novelty: For the first time, main mechanisms of interaction of the organism with VHF waves were revealed. The correlation between the state of the brain, nervous system and health and rate, frequency and duration of VHF exposure was revealed. The role of subcortical centers of reflex arch of the analyzers in formation of HNA caused by VHF radiation was shown. The causes and parameters of impairment of conditional reflexes were uncovered. The cause of pathology in unconditional reflexes(disorders of nutritional behavior, defense behavior, emotions, memory, libido) was established. For the first time, it was shown that VHF field affected in a different way the initial activity of subcortical centers participating in formation of HNA: in first centers it increased, in second - decreased, in third - remained unchanged. It was shown that VHF field damaged protein molecules and their complexes. It was established that enzyme activity changed due to the changes in the structure and properties of proteins (using the example of ACE molecule) which caused disorders in enzyme and their function renewal and promoted development of dystrophy in organoids and cells as a whole. The changes in neuroglial, nuclear-plasmatic and intranuclear relations, enlargement of neuron size, the changes in the shape, staining intensity, vacuole appearance in the cell, destruction of the cell outline were shown. Threshold doses of VHF field were determined. The ideas about the peculiarities of etiology of UHF brain pathology were formed, the concepts of dose, dose-effect, dose-time-effect, dose-frequency-effect were substantiated. The dynamics of dystrophic changes in the nerve cells followed by death (lysis) was investigated. Three periods of this pathology were discovered as well as their signs and leading mechanisms of the pathogenesis were established. A concept of biological action of UHF radiation on the human organism was worked out. Degree of introduction: The findings of the research were introduced to the teaching process of Bukovinsky Sate Medical University, Donetsk State Medical University, the work of Institute of Hygiene and Medical Ecology (Academy of Medical Science of Ukraine). Area (sphere) of application: medicine, pathological physiology
