Limanskii A. Modification and functionalization of probes and substrates for nanobiotechnology.

Objects: modified and functionalized by biopolymers probes and substrates for atomic force microscopy, immobilization processes on their surfaces. Goals: development of nanobiotechnological approaches to visualization and manipulation by single molecules and cells and determination of pathways and molecular mechanisms of supercoiled DNA molecules for immobilization onto substrate. Methods: atomic force microscopy, force measurements, polymerase chain reaction, computer analysis. Results: compaction stages of single supercoiled DNA molecules were visualized. Compressed DNA form was discovered. New decision for the problem of development of amino probes (as a biosensor components) and amino mica with defined surface properties (as a substrate for immobilization of biopolymers) by modification in vapours was found for nanobiotechnological studies. Introduction: technology of probe modification in vapours of amino silane derivative can be used for production of amino probes for atomic force microscopy andfor their following functionalization by biopolymers. Employment: the results of the compaction of single supercoiled DNA molecules can be used for undergraduate students educational programs on the biophysics, microbiology, molecular biology, nanobiology and nanobiotechnology.