Drobyk N. Physiological, biochemical and genetic bases of some Gentiana species biotechnology

The object - culturing in vitro plants of Gentiana genus from Ukrainian flora; aim is to develop the scientific foundations of biotechnology for in vitro rearing of plants from Gentiana genus of Ukrainian flora; methods provide manipulations with plant tissue and organ cultures, spectrophotometric and chromatographic techniques, cytogenetic analysis, electrophoresis of DNA in agarose gel, blot-hybridization, RAPD-PCR procedure, statistical methods; novelty: for the first time for plant species of Gentiana genus from Ukrainian flora based on study of their physiologically-biochemical and genetic peculiarities there were developed and scientifically substantiated technologies of their growing in vitro; results: there were developed methods of microclonal propagation, generation and prolong maintenance of tissue culture on various substrates, culture of isolated gentian roots, there were conducted their complex physiologically-biochemical and genetic researches; field of knowledge is plant biotechnology.