Sergeyeva T. Development and investigation of polymers-biomimics for sensor technology and solid-phase extraction

The object - processes of recognition of environmental pollutants and food toxins by polymers-biomimics, synthesized according to the method of molecular imprinting in the form of membranes and thin films. The aim - development of the scientific and technological basis of development of highly-stable counterparts of receptor sites of biomacromolecules in the structure of polymer membranes and thin films as well as their application in biosensor technology and solid-phase extraction. Methods - conductometry, impedance spectroscopy, fluorimetry, amperometry,scanning electron microscopy, the method of Bunauer-Emet-Teller, dynamical mechanical thermoanalysis, wide-angle and small-angle X-ray difraction, biochemical methods, solid-phase extraction, computational modeling, statistical methods. Results and novelty - for the first time the new principles and universal methods of synthesis of counterparts of both receptor and catalytic sites of biomolecules in the structure of polymer membranes and thin films were developed. The laboratory prototypes of electrochemical and optical biosensor devices were developed on their basis for the detection of environmental pollutants and food toxins (detection limits 1-100 nM). For the first time the structure of receptor binding sites in the MIP membranes was investigated and optimized using the methods of computational modeling. The structure of polymers-biomimics was investigated using modern physico-chemical methods.