Romanovska I. Biotechnological aspects of immobilized on polymeric supports biologically active proteinaceous substances creation.

In the dissertation are developed biotechnological approaches and methods of BAS stabilization by immobilization on natural and synthetic polymeric supports, for creation of potential preparations for wounds and burns therapy, substitutive therapy of digestion deficiency, allergic diseases diagnostics, biocatalysts of phenolic pollutants oxidation for elimination from solutions and waste waters. The principles of highly active, stable immobilized albumines, hydrolytic enzymes, allergens, of prolonged action due to noncovalent interactions with polymeric supports and their modified forms, shaped in woven, insoluble hydrogel polymeric wound dressings, polymeric films, beads and powders for potential usage in medicine, are proposed. Due to papain, trypsin and alkaline protease activity substrate inhibition, the 0,35 - 0,90 % casein solution usage recommended. By the nonvalence and valency interactions of isolated horseradish peroxidase with polymeric matrices the highly active stable biocatalysts of repeated usage for phenolic pollutants oxidation are developed. The QSAR/QSPR-models, which allows to predict the level and rate of new phenolic horseradish peroxidase substrates bioconversion are proposed. For the some of immobilized preparation the laboratory regulations and AND proposals are compiled; ophthalmotoxicity was studied. The horseradish peroxidase entrappment method in hydrate cellulose membrane was approved for quantitative phenols removal from the company "TeploService" waste waters.