Tkachuk O. Pathogenesis of immune disturbances in rats with streptozotocin-induced diabetes mellitus complicated by ischemic-reperfusing damage of the brain

The dissertation presents the results of an investigation of the pathogenesis of immune dysfunction which arises in male rats with experimental diabetes mellitus complicated by ischemic-reperfusing damage of the brain. It has been shown that diabetes of four-month duration as well as ischemia-reperfusion of the brain in the control animals and thous with diabetes are characterized by a shift of the pro-/antiapoptotic interrelations towards a prevalence of the antiapoptotic component, however, based on the degree of the expression of the nuclear antigen of cellular proliferation PCNA the deficit of thymocyte apoptosis in case of ischemia-reperfusion of the brain in control rats arises against a background of an enhancementof their proliferative activity, whereas in animals with diabetes mellitus – against a background of suppressed proliferation of the maturest functionally active classes of cells. Dysbacteriosis of the small intestine is formed in all the animals of all the experimental groups with the highest marked character in case of combined diabetes and ischemia-reperfusion of the brain. The presence of common manifestations of the immune dysfunction with different experimental interferences may be explaind by the fact that ischemia-reperfusion of the brain in the rats without diabetes causes destructive changes of the pancreatic islets, identical, although less essential, than in the rats with diabetes mellitus. The presence of specific immunological disturbances under the condition of diabetes mellitus, ischemia-reperfusion of the brain in the control animals and the animals with diabetes mellitusis corroborated by modification of the density and functional activity of various types of antigenpresenting insulinexpressing cells.