Mykhailichenko V. Role of isogenic cellular cardiomyoplasty in myocardial infarction (experimental-clinical study)

Study subject is ischemic heart disease, heart remodeling in myocardial infarction against the background of cellular cardiomyoplasty; study object is to substantiate the effectiveness of isogenic cellular cardiomyoplasty in myocardial infarction during the experiment basing on the complex functional and morphologic studies as well as the reasonability of its application in clinical practice; study methods are biochemical, electrocardiographic, ultrasonic, invasive methods of heart function analysis, morphologic, immunohistochemical, electroanatomical, statistic; study novelty: the advantages of MSC transplantation are proven; the effectiveness of cellular transplantation application in comparison with the usage of G-KSF is established; the possibility of direct migration and homing of isogenic mesenchimal stem cells in the damaged myocard by intravenous injection and their further differentiation in the endotheliocytes and fibroblasts is confirmed; the neoangiogenesis phenomenon in myocardial infarction area after the mesenchimal stem cells transplantation is proven; the advantages of intravenous transplantation method are proven; the dependence of cellular transplantation effectiveness from the time of its implementation is established as well as are proven the advantages of early cellular material introduction; the effects of mesenchimal stem cells transplantation during the myocardial infarction in rats by experiment are established; in the clinic were devised and tested the methods of stem cells self-grafting of bone marrow in refractory stenocardia therapy; it was carried out the elaboration and implementation of electrophysiological and electroanatomical diagnostic methods of ischemic myocard with the help of NOGA XP system as well as it was implemented the method of autologous cell transplantation; it was introduced in the clinical practice of State Institution "Institute of Urgent and Recovery surgery n.a. V.K.Gusak of Academy of Medical Sciences of Ukraine", in the educational process namely in the lecture course and practical exercises as well as it is used in the experimental scientific researches in the pathophysiology department, general practice department namely department of family medicine of Internship and Advanced Education Faculty, in surgical department n.a. V.M. Bohoslavskyy of Donetsk National Medical University n.a. M.Gorkyy of Health Ministry of Ukraine; in pathological physiology departments of State Medical University in Zaporozhye of Health Ministry of Ukraine, in State Institution "Lugansk State Medical University" of Health Ministry of Ukraine, in Odessa National Medical University of Health Ministry of Ukraine, in Ivano-Frankovsk National Medical University of Health Ministry of Ukraine and in State Institution "Crimean State Medical University n.a. S.I. Georgievskyy" of Health Ministry of Ukraine; application area is scientific and pedagogic processes of pathological physiology departments in medical educational institutions and treatment-and-diagnostic process of cardiosurgery and therapeutic departments.