Pylypenko L. Phytosanitary control of quarantine and potentially dangerous nematode species in Ukraine: a conceptual framework

A conceptual framework for phytosanitary control of quarantine and potentially dangerous nematode species in Ukraine was developed. It considers pest risk analysis, systematic approaches towards phytosanitary monitoring and complex application of molecular methods to nematode identification, virulence characterization and recapitulation of introduction events. Pest risk analysis for the potentially dangerous nematode species was conducted. The probability of quarantine and potentially dangerous nematode species introduction with imported commodities was studied. Phytosanitary measures to prevent this introduction were elaborated. The spread of populations of potato cyst nematode (PCN) in the Ukraine were characterized on a basis of morphological examination, molecular identification and biotest. The virulence characteristics of Globodera pallida populations of different origins and selection on nematode resistance sources were obtained. A phylogenetic analysis based on rDNA ITS, the chorismate mutase and mitochondrial cytochrome b gene sequences and six microsatellite loci genotyping data for Globodera pallida populations of different origins was conducted. A quantitative PCR assay was developed for Globodera pallida and Globodera rostochiensis detection and identification in soil samples. cDNA library obtained from second-stage juveniles of Globodera pallida was sequenced and the Globodera pallida fosmid library was screened for parasitism genes. Wild potato species and breeding material were assessed for PCN resistance by mean of a biotest and the genetic markers.