Todosiichuk T. Polyalternative biotechnology of the antiseptic preparations on the base of microbial bacteriolysin

The thesis is devoted to the scientific grounding and development of polyalternative biotechnology of multivariate antiseptic preparations based on bacteriolysin and other antimicrobial products of the selected producer Streptomyces albus UN44. The scheme of the supporting selection of producers, was proposed, the complex taxonomic analysis and reidentify the culture was conducted. The ability of the culture to synthesize complex antifungal antibiotics was detected for the first time. The main antibiotic compounds of the culture were investigated and identified as bis (2-ethylhexyl) phthalate and 3-O-metylcyclopolic acid. There was modified and optimized the composition of the nutrient medium, that that does it cheaper in 1,5-2 times and the increase of the biosynthetic activity of the culture up to 8000-9000 IU/cm3. The increased stability and efficiency of the immobilized form of the bacteriolytic preparation from S. albus UN44 were detected, which was prepared using adsorption on aerosil silica, and the main binding mechanisms in the enzyme-carrier system were shown. The immobilized bacteriolytic preparation with activity of 100 thousand IU/g, liquid complex antiseptics Cytal, liquid and granulated preparations for plant growing Streptofungin-Fito, preparations of separate bacteriolysin of a enzyme complex and a preparation of antibiotics Streptofungin are developed. Processes of application of separate preparations as a part of compositions of household and industrial synthetic detergents, at the heart of compositions of soft and liquid forms of medical veterinary preparations, and also in processes of receiving biologically active components of microbial cells and in the food industry are developed.