Triapitsyna N. Biotechnological bases of planting material obtaining for stone fruit (Prunus spp.) and berries (Ribes spp., Rubus spp., Fragaria x ananassa) crops in Ukraine

The thesis is devoted to the development of new biotechnologies and improvement of existing ones for production of healthy planting material for six stone and six berries crops. It was created the holistic concept for providing of healthy status of planting stock material at all steps of its production and operation. The biotechnologies for screening of candidate material for nuclear stock status and maintenance of healthy plant status in prebasic collections in vitro culture conditions and in open field conditions were improved. The panels of viral pathogens for the certification of planting material of stone fruit and berry crops were updated to condition of Ukraine. The main criteria for universalization of chemotherapeutic biotechnologies, based on evaluation of rapid and prolonged phase of drug action and range between therapeutic and toxic doses were identified. The universal units («pulse» and «cascade») of chemotherapy biotechnologies in vitro culture for raspberry, gooseberry, plum, rootstock Gisela 6 explants sanitation from mono- and multivirus infections were designed. The methodical algorithm for estimating of therapeutic, toxic and genotoxic effects of antiviral drugs during the explants chemotherapy in vitro culture was created. The predictive evaluation system of asymmetric viruses distribution based on determining of theirs tendencies for co-variation, association and monoexpansion was proposed. The epidemiological strategies of PPV distribution in plantations of different stone fruits crops and the main factors that can influence them were evaluated. The preventive measures to avoid the PPV spread in orchards were scientifically proved and developed for six stone fruit crops. The economical and social effects of the implementation of the program of high-quality certified plant material production of stone fruit and berry crops in Ukraine were calculated.