Zabolotna N. Multiparameter polarization-phase methods and means of reconstruction and analysis of the polycrystalling bioilogical layers structure in the process of pathological states assessment

The object of the study is the processes of polarization-phase reproduction and analysis of the structure of optic-anisotropic fields of biological layers in the evaluation of their pathological changes; the purpose of the study is to increase the reliability of the evaluation of pathological states in the systems of polarization diagnostics of histological sections of biological tissues (biological fluid films) by developing theoretical foundations and developing the latest methods and means of multiparameter polarization-phase reproduction and analysis of the structure of polycrystalline biological monolayers and two-layer polycrystalline biological networks; research methods are based on the use of the theory of interaction of laser radiation with biological media, methods of Stokes-polarimetry, methods of statistical, correlation and fractal analysis; the theoretical results - for the first time the differentiation was proposed for groups of muiller-matrix images of histological sections of multilayered biological tissues and liquids by the values of birefringence and orientation of optical axes of their polycrystalline networks; for the first time, methods of direct reproduction and analysis of coordinate distributions, respectively, of orientation and phase parameters of the polycrystalline structure of an optically thin biological layer was proposed, for the first time the theoretical basis of the Muller-matrix reproduction of coordinate distributions of orientation and phase parameters of the structure of shielded outer biological layers of two-layer biological tissue was developed; was first proposed the concept of constructing a multiparameter polarization-phase reproduction system and analyzing the anisotropy parameters of a biological layer, which implements a set of proposed methods in providing experimental system flexibility and enhanced functional capabilities; practical results - circuit design solutions and program-algorithmic means for implementation of each of the proposed methods have been developed and a prototype system has been developed that implements a complex of polarization-phase methods of reproduction and analysis of parameters of structural anisotropy of biological layers in the evaluation of pathological states with high reliability. Degree of implementation - separate results of the dissertation work were introduced into the clinical research "Regional Bureau of Forensic Medical Examination" of the State Health Care of Chernivtsi Regional State Administration and Bukovina State Medical University (BDMU); Chernivtsi National University. Yuri Fedkovych, the Department of Laser and Optoelectronic Technology of the Vinnytsia National Technical University, the Department of Forensic Medicine and Medical Law of the BDMU introduced into the educational process. Scope - systems of diagnostics of histological sections of biological tissues and films of biological fluids.