The work is devoted to the experimental solving of a scientific problem of pharmacognostic research on the herbal sources of phenylpropanoids, scientific grounding of the choice of the objects for the research, herbal raw material standardization and development of medicinal agents for the urogenital system disorders treatment.
The analysis of medicinal agents for the treatment of abnormal uterine bleedings and urological disorders in Ukrainian pharmaceutical market by assortment structure was carried out, and market segmentation by the country of origin, origin of active pharmaceutical ingredient and form of presentation was performed. Marketing research has shown limited assortment of medical agents of herbal origin, which became the basis for the search of herbal sources of phenylpropanoids with large raw material base, as well as the creation of domestic medicinal agents with the purpose of widening the assortment of medicines in the market segment of interest.
The experimental research on the herbal sources of phenylpropanoids and other groups of biologically active compounds, which are used in the urogenital system disorders treatment: flavonoids, anthocyanidins, hydroxycinnamic acids, phenolic glycosides, organic and fatty acids, was carried out.
Experimental data of the phytochemical study of the objects of the research allowed choosing prospective raw material for the further standardization and herbal medicinal agents’ development: black currant fruits in the fresh, dried and pomace state, shepherd’s purse herb, onion husks, fresh and dried cranberry fruits. For the chosen herbal raw material types the main macro- and microscopic diagnostic features, as well as the quality parameters, were determined, which underlay the development of the national monograph “Shepherd’s Purse HerbN”, included in the SPU 2.2, and the projects of national monographs “Black currant Fruits FreshN” and “Black currant Fruits DriedN”, recommended for the inclusion into the SPU 2.4. Herbal medicinal agents on the basis of prospective raw material, containing phenylpropanoids, were developed: fresh cranberry fruits dry extract, shepherd’s purse herb thick extract, onion husks dry extract, “Oxyriburcep” species in the form of briquettes, “Riglycep” briquettes, for which the chemical composition was studied and standardization parameters were determined. The antimicrobial activity towards Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Proteus vulgaris, Bacillus subtilis, Candida albicans and anti-inflammatory activity on the model of carrageenan-induced edema of the obtained herbal medicinal agents was determined by pharmacological studies. Antihemorrhagic activity was proven for the shepherd’s purse herb thick extract.
The projects of quality control methods for “Cranberry fruits fresh”, “Cranberry fruits dried”, “Onion husks”, “Black currant pomace”, “Cranberry fruits dry extract”, “Onion husks dry extract”, “Shepherd’s purse herb dry extract”, “Oxyriburcep” species in the form of briquettes”, and “Riglycep” briquettes” have been developed, which are reproduced under industrial conditions of “Fittex” LLC. The quality control methods for the medication “Aflazin” (registration certificate № UA/11171/01/01 of 12.11.2015) and a dietary supplement “Diurol” produced by Valartin Pharma LLC, which contain the herbal sources of phenylpropanoids and are used in the urogenital system disorders treatment and prophylaxis, were developed.
