Matskevych V. Microclonal propagation of plant species in vitro and their post-septic adaptation

Based on many years of research, the dissertation provides a theoretical generalization and a new solution to the current problem of agricultural production - the use of microclonal propagation of plant species in vitro to rid various infections: bacterial, fungal and viral, preservation in the state of juvenile taking into account the specific manifestation of their biological characteristics. adaptation. The possibility of leveling apical dominance with the use of cytokinins has been proved: BAP (0.5 mg / l) against the background of IBA (0.25 mg / l). The phytotoxicity of BAP can be reduced by using gibberellins. Measures have been developed to minimize the hyperhydration of test tubes. The effect of kinetins on potato tuber formation in vitro was revealed. When cultivating potatoes, on a medium with potato starch or tuber extract, instead of sucrose and agar, it had a positive effect on the development of the root system, stolons, reduced the period from grafting to tuber formation. In potatoes, blackberries had a positive effect on plant growth and development in vitro replacement of the negligent form of iron in Murasige and Skuga by Ferrileme 4.8 Orto-Orto. Proven positive effect on rhizogenesis of hosts in vitro increase in the photoperiod from 8 to 16 hours. per day, as well as the replacement of IAA (1-5 mg / l) with IBA (1-5 mg / l). There was no significant difference in the manifestation of rooting in the host variety Patriot after the replacement of activated carbon (1.5 g / l) with wood (2.5 g / l). It was found that hydrogels and perlite were the best substrates for engraftment of cuttings during postseptic adaptation of Podolyanka potato regenerators.