The object of study: pathological changes in the connective tissue, caused by hormonal disorders of reproductive function in males. The goal of the work is: to determine the contribution of disturbance of the structural elements of connective tissue to the development of hormone-dependent pathology of the reproductive system as a basis for improving approaches to its correction in males. Research methods: physiological, histochemical, histological,ultramicroscopy, cytological, enzyme-linked immunosorbent assay, statistical. Equipment: Scales torso- ВТ-500, immunoenzyme analyzer "StatFax 2100", Goryaev camera, ЕМВ-100БР electron microscope, Mikros 400 microscope, Nicon Col Pix 4500 digital camera. The results substantiate the feasibility of clinical studies on the use of agents - stimulators of connective tissue regeneration in andrology and reproductive endocrinology and make it possible to expand the range of their medical use for androgen-dependent disorders of the spermatogenic function of testicles. The content of chondroitinsulfates in sperm plasma and glycosaminoglycans in the testes as markers of connective tissue metabolism in male rats have been determined using various experimental models of reproductive system pathology. For the first time, the contribution of the disturbunce of the structural elements of the connective tissue as one of the little-known pathogenetic links of reproductive diseases in males have been determined by experimental modeling of androgen deficiency of various origins in male rats. The modern scientific understanding of the pathogenetic aspects of the relationship of androgen-dependent male reproductive diseases with associated changes in the state of connective tissue has been expanded, which made it possible to substantiate the correction of these disorders using agents that affect the metabolism of connective tissue and the state of spermatogenesis. The work is is devoted to the actual problem of reproductive endocrinology – experimental research of the contribution of disturbance of structural elements of connective tissue as a leading link in the pathogenetic chain, which promotes the formation of hormone – dependent reproductive diseases; as well as a theoretical justification for the use of connective tissue remodulating agents for the correction of disorders of the reproductive system in males. It was found that independently of the experimental model of gonadopathy (serotonin, radiation, cytotoxic) in male rats on the background of the androgen deficiency state, there is a disturbance of the morphofunctional state of the mitochondria of testicular cells, damage to the nuclear membrane, a decrease in the content of testosterone in the blood and fructose in the seminal vesicles; a decrease in the concentration and mobility of spermatozoa and the content of connective tissue components of glycosaminoglycans in the testes and chondroitin sulfates in the sperm plasma. It was proved that in all variants of experimental gonadopathies the use of drugs that positively affect the structure of connective tissue (chondroitin sulfate and Bioglobin-U), reduces degenerative and destructive processes in the mitochondria of Leydig and Sertoli cells, significantly improves testosterone secretion and testosterone secretion in testes and chondroitin sulfates in spermoplasm, which is a necessary condition for the normalization of incretory and spermatogenic functions in the simulation of their hypofunction. It has been experimentally proven that in the conditions of the chronic prostatitis simulation, the agents that regenerate the structure of connective tissue prevent destructive changes in the acinus of the prostate, significantly increase the content of glycosaminoglycans in the seminal tubules and intertubular connective tissue of experimental animals. In the simulation of benign prostatic hyperplasia it was proved that the use of drugs that have a positive effect on the structure of connective tissue leads to a decrease in inflammation and lipid peroxidation and intensification of prostate regeneration, to remodulation of connective tissue structure by reducing fibrous infiltrates. interacinar stroma, to the restoration of stromal and epithelial tissue. The positive effect of the connective tissue component - chondroitin sulfate and its combination with Tribestan on the morphostructure of the testes and prostate, endocrine and spermatogenic function of the testicles in experimental animals with gonadopathies and chronic prostatitis can be the basis for justifying the use of agents that remodel connective tissue in the treatment of testicular and post-testicular variants of pathospermia. Implementation degree: 5 acts of implementation, 3 patents. Scope of application: biology, endocrinology, andrology, pharmacology, educational process.
