Varynskyj B. Chromatographic and mass spectrometric determination of active pharmaceutical ingredients – derivatives of 3-thio-1,2,4-triazole in medicinal products and biological objects

The dissertation is devoted to scientific and theoretical substantiation, elaboration and test of approaches concerning development of bioanalytical (metabolism, determination of residual quantities) and analytical methods of determination of 3-thio-1,2,4-triazole derivatives (API standardization) using HPLC-DMD and HPLC-MS methods in substances and drugs, validation of methods, and then apply them. Based on these approaches, a methodological basis is proposed and recommendations are made for the quantitative determination of 3-thio-1,2,4-triazole derivatives and their synthons in pharmaceutical and biological samples. The study of forced degradation of piperidinium 2-((5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol) -3-yl) acetate and morpholinium 2-((4-(2-methoxyphenyl)-5-(pyridin-4-yl)-4H-1,2,4-triazol-3-yl) thio) acetate. A pharmacopoeial standard sample of 2-((4-(2-methoxyphenyl)-5-(pyridin-4-yl) -4H-1,2,4-triazol-3-yl) thio) acetate was prepared.