Kolisnik I. Pathochemical mechanisms of structural and metabolic disorders during prolonged exposure to sodium fluoride (experimental study)

The dissertation research provides a theoretical generalization and solution of a topical scientific medical problem - structural and metabolic disorders and their mechanisms with prolonged exposure to sodium fluoride. Experimental method on laboratory animals artificially reproduced exogenous induction by chemical environmental factor - sodium fluoride and pathogenetically substantiated the principles of early diagnosis of detected changes in sodium fluoride. Based on these studies, a decrease in all protective compensatory reactions of cells in response to long-term action of sodium fluoride - violation of the reception of mediators in organs and tissues of rats, as a guarantor of functional rest of cells - levels of cAMP, cGMP and calcium ions. Decreased enzyme complexes of the respiratory chain, enzymatic activity of hepatocyte microsomes, imbalance of bioenergetic processes - free radical reactions and LPO - due to decreased respiratory activity of hepatocyte mitochondria and impaired liver detoxification function. The results of comprehensive research allowed to formulate a deep and comprehensive picture of the long-term (60 days) effect of sodium fluoride on metabolic processes, substantiated criterion and significant indicators of early diagnosis of pathological conditions and a conceptual model of pathophysiological mechanisms of structural and metabolic disorders. It is established that the leading pathogenetic chains of dystrophic and destructive processes in the subtoxic action of sodium fluoride are the activation of free radical processes, peroxidation of lipids, proteins, nucleic acids; depletion of the antioxidant system; inhibition of bioenergy, tissue respiration and oxidative phosphorylation, which form the development of molecular membrane pathology.