Chornopyshchuk R. Multimodal principles of care for patients with burns: prevention, diagnosis, treatment (experimental-clinical study)

The dissertation work is experimental and clinical in nature and is devoted to improving the results of providing care to patients with burn injuries by preventing the progression of burn damage, improving diagnostic criteria, developing comprehensive treatment principles using multi-vector antimicrobial agents, the latest wound dressings, original physiotherapeutic and surgical methods. A new direction in complex treatment of burn wounds has been developed in the work, as a result of which the following scientific data were obtained: an original method of simulating scalds in rats with the possibility of simple, affordable and reliable reproduction was developed; scientific data on the qualitative and quantitative composition of microorganisms isolated from burn wounds were updated; a slightly adhesive composite polymer material based on poly-(2-hydroxyethyl methacrylate), modified with a pore former with the addition of antimicrobial agents (Ukraine patent No. 125133 dated January 12, 2022) was developed; the value of the indicator of microcurrent generation without external power supplies in the interelectrode space of a closed circuit of energy circulation was established to be dependent not only on the electrochemical nature of the electrodes, but also on the type of microorganisms beneath; the ability of low-intensity current without external power supplies was confirmed to not only suppress the growth of microorganisms, to increase their sensitivity to antibacterial drugs, but also to suppress their biofilm-forming properties; safety and biological compatibility of the material made from porcine peritoneum, its effectiveness compared to other materials of natural origin was experimentally confirmed; according to the results of a microbiological study in "in vitro" experiments, high antimicrobial activity of a multicomponent sorption nanocomposite based on silica, polymethylsiloxane, decamethoxine, metronidazole was confirmed not only on museum strains of microorganisms, but also on isolates obtained from patients with burns; positive effect of injecting autologous platelet-rich plasma on the period of restoring skin integrity and accelerating the eradication rate of wound infection pathogens has been scientifically proved; the suggested technology for restoring skin integrity after burns in rats by means of autologous microskin grafting was experimentally developed and scientifically confirmed.