Soldatkin O. Development of scientific and technological basis for creation of electrochemical biosensory for medicine, biotechnology and environmental control

There is development of scientific and technological basis for creation of potentiometic and conductometric enzyme and cell sensor in the thesis/Series of the biosensors were developed for direct and inhibitor analysis of important metabolytes and toxines (glucose, uren, acetyl-and butirylchoime, penicilline, peptide substrates, etyl- and metylalcohol, formaldehyde, hypochiorite, phosphoorganic pesticides, heavy metal ijns).The labarotory prototypes of biosensors were created and tested for analysis of substrates and inhibitors (toxic agents) in model solution and real biologycal samples.Analytical characteristics of developed biosensors and optimal condition for their performance and storage were studied in detalls.Coparetive analysis of enzyme and cell biosensors was done.The investigations were directed on the detalled analysis of the ways to improve the analytical characteristics of the biosensors developed too.It was shown that the presence of additional charged lays of the sensor response on the bulfer capacity, ionic strength. pH value and protein concentration of the analysed sample, extends of dynamic range of analyte-concentration.