The dissertation is devoted to the study of the structure peculiarities of the ovaries of the rats’ offspring after experimental modeling of the progesterone effect by introducing a hormone solution to females from 15th to 18th days of pregnancy using anatomical, morphometric, histological, immunohistochemical and statistical methods of study.
Using anatomical and morphometric methods the dynamics of mass-growth parameters of animals were investigated. During the first three months of postnatal development, the weight of control and experimental animals gradually increased along with the observation time increase, reaching maximum values by the end of the study period. In animals born from females with impaired hormonal status, from 1st to 9th days of age, inclusive, body weight is likely to exceed the control values, on the 14th day the difference between the mass indexes is balanced. In the future, from the 30th day until of the end of the observation period for the body mass indexes of the animals of the experimental group, there is a characteristic tendency to the significant lag from the controls (102,47 ± 2,11 g та 123,86 ± 1,76 g, respectively). Investigating the dynamics of occipital-coccygeal distance in the offspring of animals, a gradual increase in this indicator was found in all study groups of animals with maximum values after the ninety-day life, with no significant differences between the groups – 10,9 ± 0,15 cm in control animals and 10,3 ± 0,17 cm in experimental animals, respectively. However, at day 14th in experimental rats, the body length index was significantly higher than the control values (5,58 ± 0,13 cm and 5,18 ± 0,23 cm, respectively). In the group of experimental animals at 1st and 9th days of postnatal life after birth, a significant excess of body mass index (BMI) was observed over the control values. On the 45th day of life, on the contrary, there was a significant lag in the BMI in the experimental group. However, from the 60th day to the end of the observation period, the BMI difference in the animal groups was balanced (1,0 ± 0,02 g/cm2 and 0,96 ± 0,07 g/cm2, respectively).
It was first established that the offspring of animals born to females receiving progesterone injections from 15th to 18th days of gestation were characterized by impaired process of formation of a pool of primordial follicles. At 1st day after birth in experimental animals, the relative area occupied by the formed primordial follicles is smaller than in the control animals (34,34 ± 1,14 % vs. 36,45 ± 0,67 %, respectively). However, there was no significant difference between clusters relative indices in the groups. On the 3rd day of life in the group of animals after progesterone exposure during the prenatal period, unlike control rats, non-destructive clusters were present, occupying 4,31 ± 0,22 % of organ area, respectively. Hence, in all the study groups of animals, the pool of primordial follicles has gradually decreased, reaching 2,62 ± 0,11 % in experimental rats by the end of the observation period, which is significantly lower than in control rats (4,0 ± 0,57 %, respectively). A similar tendency to decrease the relative area was observed for primary follicles.
