Dzhivak V. The pathogenetic rationale for the use of platelet-rich blood plasma and stem cells for traumatic muscle injuries correction

The thesis presents a theoretical generalization and a new solution of the current scientific problem, which is manifested in the relationship of morphological changes in skeletal muscle tissue with changes at local and systemic levels under experimental traumatic muscle injury and platelet-rich correction. plasma (PRP) and mesenchymal stem cells (MSCs) in the early and late post-traumatic periods, and studying the effect of PRP on the proliferative activity of fibroblasts, myogenic cells and mesenchymal stem cells of rat umbilical cord in vitro. In the dissertation work the influence of PRP on the proliferative activity of fibroblasts, myogenic cells and mesenchymal stem cells of rat umbilical cord in vitro was studied for the first time. It was found that platelet-rich plasma has a pronounced mitogenic effect on mesenchymal cells, a significant anti-inflammatory effect. has a pronounced stimulating effect on the proliferative activity of fibroblasts, but less effective in the case of cell culture of mesenchymal stem cells. It was found time that under the conditions of experimental correction with the use of PRP there is an inhibition of lipid peroxidation and an increase in the activity of enzymes of the antioxidant system. Muscle injury simulations were demonstrated to be accompanied by an increase in the concentration of pro-inflammatory cytokines IL-1β TNF-α IL-6 with maximum growth on the first day, a steady increase during days 7-th-14-th, and a decrease to 21-st.days Traumatic muscle injuries have been shown to cause endotoxicosis, which is manifested by the accumulation of endotoxins in the body, as evidenced by significant changes in endogenous intoxication: erythrocyte and leukocyte indices of intoxication and the content of medium weight molecules. Animals with experimental traumatic thigh muscle injury were found to have an intense increase in blood proteolytic activity with less pronounced increase in antiprotease activity, as well as an increase in lysosomal membrane permeability, leading to a significant imbalance in the protease / antiprotease system and an increase in proteolysis index. А set of muscle disorders after injury was shown histologically and morphometrically, and the anti-inflammatory and regenerative effects of platelet-enriched plasma and mesenchymal stem cells were demonstrated. In the simulation of traumatic muscle injury, it was found that on the 1st day of the experiment, the organs we studied developed dystrophic changes in all epithelial components, but they are most pronounced in the liver and lungs. The use of PRP and MSC has a different degree of protective effect from the 1st day of use. The use of PRP has a predominantly local effect to correct the healing of traumatic muscle damage, improves the recovery of structures in the wound area and has a superficial effect on the recovery of the epithelial component of parenchymal organs, mainly in the long term.